Standardization of serum ceruloplasmin concentrations in international enzyme units with o-dianisidine dihydrochloride as substrate.
نویسندگان
چکیده
We describe a method for calculating the absorptivity (in terms of substrate consumed) of the colored solution obtained when o-dianisidine dihydrochloride is oxidized by ceruloplasmin. By oxidizing o-dianisidine dihydrochloride with known amounts of hydrogen peroxide we could determine that the molar reacting ratio of o-dianisidine to hydrogen peroxide is 2:1. So calculated, absorptivity is 9.6 ml zmor1 cm’, the figure used to estimate ceruloplasmin oxidase activity in terms of International Units.
منابع مشابه
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 20 12 شماره
صفحات -
تاریخ انتشار 1974